Real Time PCR Diagnostic Kit


1. Utilizing the TaqMan probe technology, TBC developed a series of real-time PCR products. TaqMan probes are hydrolysis probes, which can increase the specificity of traditional real-time PCR assays. The TaqMan probe principle relies on the 5´–3´ nuclease activity of Taq polymerase to cleave a dual-labeled probe during hybridization to the complementary target sequence and fluorophore-based detection. As in other real-time PCR methods, the resulting fluorescence signal permits quantitative measurements of the accumulation of the product during the exponential stages of the PCR; however, the TaqMan probe significantly increases the specificity of the detection. TaqMan probes were named after the videogame PacMan (Taq Polymerase + PacMan = TaqMan) as its mechanism is based on the PacMan principle.

2. TBC produces a wide variety of real-time PCR kits including respiratory and intestinal pathogenic bacteria diagnostic products. 

3. Except for the sequences published on the GenBank, we sequenced a wide variety of strains. Blasting with GenBank and TBC’s database, all the primers are well designed, which ensures the extremely high specificity. 

4. All the products were verified by conservative and sensitivity experiments. Utilizing TBC’s rich strain resources, each kit was validated by more than 20 standard and clinical strains to ensure the conservation. Normally, 40 closely related strains were tested to ensure the specificity of the kits. These quality control procedures sufficiently mark down the false positive and false negative result.

5. High repeatability: All the products are validated by a large variety of strains, which ensures the 100% repeatability.

6. We provide high sensitive and specific detection. 103cfu/ml sample is required for a typical experiment.

7. All ready to use reagents including controls hence no errors while reagents preparation.

8. Total amplification time required is least possible, which normally takes 3-4 hours.  

9. 6 months shelf life leading to no wastage of reagents and suitable for smaller laboratories.


Legionella pneumophilia Real Time PCR Diagnostic Kit  
Legionella Real Time PCR Diagnostic Kit  
Streptococcus pneumonia Real Time PCR Diagnostic Kit
Streptococcus Real Time PCR Diagnostic Kit
Bacillus cereus Real Time PCR Diagnostic Kit
Escherichia coli O157 Real Time PCR Diagnostic Kit
Salmonella Real Time PCR Diagnostic Kit
Shigella Real Time PCR Diagnostic Kit
Staphylococcus aureus Real Time PCR Diagnostic Kit
Vibrio cholera O1 Real Time PCR Diagnostic Kit
Vibrio cholera O139 Real Time PCR Diagnostic Kit
Enterohemorrhagic Escherichia coliEHECReal Time PCR Diagnostic Kit
Pseudomonas aeruginosa Real Time PCR Diagnostic Kit
Listeria monocytogenes Real Time PCR Diagnostic Kit
Campylobacter jejuni Real Time PCR Diagnostic Kit
Enterobacter sakazakii Real Time PCR Diagnostic Kit